abstract：:The enteric nervous system (ENS) of the larval moth Manduca sexta consists of two small ganglia and several nerve networks that lie superficially along the alimentary tract. Within this system are approximately 600 neurons that exhibit a spectrum of biochemical and morphological characteristics and that express these ...

journal_title：Developmental biology

authors： Copenhaver PF,Taghert PH

更新日期：1989-01-01 00:00:00

abstract：:Single cells from 512-cell Xenopus embryos, totally labeled with intracellular horseradish peroxidase, were transplanted orthotopically (from either dorsal or lateral marginal zone) or heterotopically (between these two marginal zones) to unlabeled host embryos at the 512-cell stage. At tailbud stage 23 the locations,...

journal_title：Developmental biology

authors： Jacobson M,Xu WL

更新日期：1989-01-01 00:00:00

abstract：:The formation of primary mesenchyme cells in euechinoid sea urchin embryos involves the transformation of 32 epithelial cells into mesenchymal cells in a process referred to as ingression. The mechanism that drives this epithelial-mesenchymal transformation has yet to be identified. Previous studies (J. R. Gibbins, L....

journal_title：Developmental biology

authors： Anstrom JA

更新日期：1989-01-01 00:00:00

abstract：:We describe the lineage and morphogenesis of neural plate cells in the ascidian, Ciona intestinalis, from reconstructed cell maps of embryos at 12-min intervals during and after neurulation, between 31 and 61% of embryonic development. Neurulation commences in a posterior to anterior wave following in the wake of the ...

journal_title：Developmental biology

authors： Nicol D,Meinertzhagen IA

更新日期：1988-12-01 00:00:00

abstract：:Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...

journal_title：Developmental biology

authors： Kawamura M,Urist MR

更新日期：1988-12-01 00:00:00

abstract：:Extracellular cAMP induces the intracellular synthesis and subsequent secretion of cAMP in Dictyostelium discoideum (relay). cAMP relay was strongly diminished in mutant HB3 which shows abnormal development by making very small fruiting bodies. Extracellular cAMP binds to receptors on the surface of mutant cells and i...

journal_title：Developmental biology

authors： Kesbeke F,Van Haastert PJ

更新日期：1988-12-01 00:00:00

abstract：:To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...

journal_title：Developmental biology

authors： Donahue SP,Wood JG,English AW

更新日期：1988-11-01 00:00:00

abstract：:The accumulation of the mRNAs from four Strongylocentrotus purpuratus actin genes (the single muscle gene M, and three cytoskeletal genes CyI, CyIIIa, and CyIIIb) and of transcripts from an RNA polymerase III-transcribed repeated sequence family (SURF1) was followed throughout the early development of hybrid embryos o...

journal_title：Developmental biology

authors： Bullock BP,Nisson PE,Crain WR Jr

更新日期：1988-11-01 00:00:00

abstract：:Cell surface and cytoplasmic polarity is exhibited by the blastomeres of mouse preimplantation embryos following compaction at the 8-cell stage of cleavage. It has been hypothesized that cytoplasmic polarity is initiated by plasma membrane functions of polar blastomeres that are absent from apolar blastomeres. To test...

journal_title：Developmental biology

authors： Wiley LM,Obasaju MF

更新日期：1988-11-01 00:00:00

abstract：:Cyclic protein-2 (CP-2) is secreted in vitro in substantial amounts by mature rat Sertoli cells in intact Stage VI and Stage VII seminiferous tubules. This stage-dependent secretion has led us to postulate that the biosynthesis of this molecule is stimulated by germ cells at a specific state of development. In order t...

journal_title：Developmental biology

authors： Wright WW

更新日期：1988-11-01 00:00:00

abstract：:During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as...

journal_title：Developmental biology

authors： Paine R,Ben-Ze'ev A,Farmer SR,Brody JS

更新日期：1988-10-01 00:00:00

abstract：:An anterior-posterior concentration difference of fibronectin associated with the endoderm in early chick embryos has been implicated in the directional migration of precardiac mesoderm cells. We have examined the effect of increasing concentrations of an antibody to fibronectin (FN) to test the essentiality of FN to ...

journal_title：Developmental biology

authors： Linask KK,Lash JW

更新日期：1988-10-01 00:00:00

abstract：:The expression of the neural cell adhesion molecules N-CAM and L1 was investigated in the olfactory system of the mouse using immunocytochemical and immunochemical techniques. In the olfactory epithelium, globose basal cells and olfactory neurons were stained by the polyclonal N-CAM antibody reacting with all three co...

journal_title：Developmental biology

authors： Miragall F,Kadmon G,Husmann M,Schachner M

更新日期：1988-10-01 00:00:00

abstract：:Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...

journal_title：Developmental biology

authors： Rizzino A,Kuszynski C,Ruff E,Tiesman J

更新日期：1988-09-01 00:00:00

abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...

journal_title：Developmental biology

authors： Bates WR

更新日期：1988-09-01 00:00:00

abstract：:Cytoskeletal intermediate filaments were studied in muscular dysgenesis (mdg) and tetrodotoxin-treated inactive mouse embryo muscle cultures during myofibrillogenesis. Both muscular dysgenesis and tetrodotoxin-treated muscles are characterized in vitro by a total lack of contractile activity and an abnormal developmen...

journal_title：Developmental biology

authors： Tassin AM,Pinçon-Raymond M,Paulin D,Rieger F

更新日期：1988-09-01 00:00:00

abstract：:Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...

journal_title：Developmental biology

authors： Endo Y,Lee MA,Kopf GS

更新日期：1988-09-01 00:00:00

abstract：:Carbohydrate chains on the t12 mutant embryos were analyzed. No abnormalities of the synthesis of the carbohydrate chains were observed in the t12 homozygotes until the late morula stage, when radiolabeled carbohydrate chains were analyzed by Sephadex G-50 column chromatography. Furthermore, polarization of the Con A ...

journal_title：Developmental biology

authors： Iwakura Y,Nozaki M

更新日期：1988-08-01 00:00:00

abstract：:We have examined the morphology of fetal rat sympathetic neurons grown in serum-free medium in the absence of nonneuronal cells. Because cell density can affect phenotypic expression in vitro, the morphological analysis was subdivided into the study of isolated neurons (neurons whose somata were at least 150 micron fr...

journal_title：Developmental biology

authors： Bruckenstein DA,Higgins D

更新日期：1988-08-01 00:00:00

abstract：:cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating...

journal_title：Developmental biology

authors： Wang M,Van Haastert PJ,Devreotes PN,Schaap P

更新日期：1988-07-01 00:00:00

abstract：:Monoclonal antibodies (mAbs), electrophoresis, immunoblotting, and immunohistochemistry were used to determine the molecular properties of cardiac myosin heavy chain (MHC) isoforms and the regions of the developing chicken heart in which they were expressed. Adult atria expressed three electrophoretically distinct MHC...

journal_title：Developmental biology

authors： Evans D,Miller JB,Stockdale FE

更新日期：1988-06-01 00:00:00

abstract：:We have tested the role of proteoglycans in the development of feather pattern by culturing 7-day-old embryonic chick skins on medium containing para-nitrophenyl-beta-D-xyloside (2 mM). Xylosides compete with core proteins of proteoglycans by acting as exogenous acceptors for the synthesis of glycosaminoglycans leadin...

journal_title：Developmental biology

authors： Goetinck PF,Carlone DL

更新日期：1988-05-01 00:00:00

abstract：:Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...

journal_title：Developmental biology

authors： Gipson IK,Spurr-Michaud SJ,Tisdale AS

更新日期：1988-04-01 00:00:00

abstract：:A functional ribosomal protein mRNA, encoding the 60 S subunit protein L1, has been synthesized in vitro using bacteriophage SP6 RNA polymerase. This mRNA directs the synthesis of a product indistinguishable from L1 protein purified from Xenopus ovarian ribosomes. Our results show that L1 synthesis in stage VI oocytes...

journal_title：Developmental biology

authors： Baum EZ,Hyman LE,Wormington WM

更新日期：1988-03-01 00:00:00

abstract：:Expression of the c-abl proto-oncogene in the mouse testis is characterized by the production of a unique 4.7-kb transcript present in germ cells that have entered the haploid phase of spermatogenesis. A similar developmental stage specificity of expression is observed for a member of the 70-kDa heat-shock protein (hs...

journal_title：Developmental biology

authors： Zakeri ZF,Ponzetto C,Wolgemuth DJ

更新日期：1988-02-01 00:00:00

abstract：:The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...

journal_title：Developmental biology

authors： Ettensohn CA,McClay DR

更新日期：1988-02-01 00:00:00

abstract：:Mouse sperm surface galactosyltransferase (GalTase) mediates fertilization by binding to its appropriate glycoconjugate substrate in the egg zona pellucida. GalTase is present throughout all stages of spermatogenesis, during which time it redistributes within the plasma membrane from a uniform, diffuse distribution on...

journal_title：Developmental biology

authors： Scully NF,Shur BD

更新日期：1988-01-01 00:00:00

abstract：:Type II collagen is a major component of cartilage extracellular matrix. Differentiation of mesenchyme into cartilage involves the cessation of type I collagen synthesis and the onset of type II collagen synthesis. Solution hybridization of mRNA isolated from chick limb buds with a cDNA probe to type II collagen mRNA ...

journal_title：Developmental biology

authors： Swalla BJ,Upholt WB,Solursh M

更新日期：1988-01-01 00:00:00

abstract：:Experiments in which the developing gut of avian embryos was back-transplanted to permit the bowel to interact with the developing neural tube were undertaken. Segments of intestine from 4-day quail embryos were implanted between the somites and neural tubes of chick embryos of 7 to 24 somites. The spinal cord respond...

journal_title：Developmental biology

authors： Rothman TP,Gershon MD,Fontaine-Pérus JC,Chanconie M,Le Douarin NM

更新日期：1987-12-01 00:00:00

abstract：:Autoradiography has been used to confirm and to extend previous microspectrophotometric studies (Doerder and DeBault, 1975) on the timing of DNA synthesis during conjugation in Tetrahymena thermophila. The majority of DNA synthesis occurs at the expected periods preceding gamete formation and the two postzygotic divis...

journal_title：Developmental biology

authors： Allis CD,Colavito-Shepanski M,Gorovsky MA

更新日期：1987-12-01 00:00:00

abstract：:The effect of epidermal growth factor (EGF) on cellular differentiation of the neonatal mouse mandibular incisor was examined autoradiographically using tritiated thymidine ([3H]TDR) and tritiated proline ([3H]PRO). On days 0 (day of birth), 1, and 2, EGF was administered (3 micrograms/g body wt) sc to neonates. Mice ...

journal_title：Developmental biology

authors： Topham RT,Chiego DJ Jr,Gattone VH 2nd,Hinton DA,Klein RM

更新日期：1987-12-01 00:00:00

abstract：:Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertil...

journal_title：Developmental biology

authors： Prigent C,Maniey D,Lefresne J,Epel D,Signoret J,David JC

更新日期：1987-11-01 00:00:00

abstract：:The relative transcription of three unlinked actin genes of the sea urchin Strongylocentrotus purpuratus was measured in isolated nuclei, at several stages during embryonic development. Transcription of two cytoskeletal actin genes, CyI and CyIIIa, was first detected in 64-128 cell embryos. At the early stages, the Cy...

journal_title：Developmental biology

authors： Hickey RJ,Boshar MF,Crain WR Jr

更新日期：1987-11-01 00:00:00

abstract：:The sizes of amino acid pools in growing Xenopus laevis oocytes have been measured. The total free amino acid content per oocyte increases nearly 25-fold during oocyte growth. Together, glutamic acid and aspartic acid account for approximately 59-75% of the total amino acid pool in Xenopus oocytes. On the other hand, ...

journal_title：Developmental biology

authors： Taylor MA,Smith LD

更新日期：1987-11-01 00:00:00

abstract：:An immunocytochemical study of cholecalcin (28-kDa calcium-binding protein, CaBP, calbindin) was carried out during the development of the rat hippocampus. In normal animals, the protein appeared from Postnatal Day 3 in the granule cells of the dentate gyrus and from Day 5 in the CA1-CA2 pyramidal cells of Ammon's hor...

journal_title：Developmental biology

authors： Rami A,Bréhier A,Thomasset M,Rabié A

更新日期：1987-11-01 00:00:00

abstract：:During development of Dictyostelium discoideum, the cellular specific activity of beta-glucosidase increases before aggregation, declines to low levels during pseudoplasmodium formation, and increases rapidly during culmination. In addition, two electrophoretically distinct isozymes of beta-glucosidase are present at ...

journal_title：Developmental biology

authors： Golumbeski GS Jr,Dimond RL

更新日期：1987-10-01 00:00:00

abstract：:The HNK-1 antibody recognizes a carbohydrate moiety that is shared by a family of cell adhesion molecules and is also present on the surface of migrating neural crest cells. Here, the effects of the HNK-1 antibody on neural crest cells were examined in vitro and in vivo. When the HNK-1 antibody was added to neural tub...

journal_title：Developmental biology

authors： Bronner-Fraser M

更新日期：1987-10-01 00:00:00

abstract：:In this investigation, we characterize the embryonic and adult actins and describe the embryonic expression of a muscle actin in the ascidian Styela. Two-dimensional polyacrylamide gel electrophoresis showed that embryos, tadpole larvae, and adult organs contain three major and two minor isoforms of actin. Two of the ...

journal_title：Developmental biology

authors： Tomlinson CR,Bates WR,Jeffery WR

更新日期：1987-10-01 00:00:00

abstract：:The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yo...

journal_title：Developmental biology

authors： Stamatoyannopoulos G,Constantoulakis P,Brice M,Kurachi S,Papayannopoulou T

更新日期：1987-09-01 00:00:00

abstract：:A rapid, gentle technique is described for the isolation of nuclei from sea urchin embryos. Using this technique, we have analyzed the synthesis and accumulation of nonhistone nuclear proteins during sea urchin development by two-dimensional gel electrophoresis. Most nuclear proteins fall into one of three patterns of...

journal_title：Developmental biology

authors： Servetnick MD,Wilt FH

更新日期：1987-09-01 00:00:00